ABSTRACT
Background: The interplay between inflammation and lipids has recently been the focus of research aimed at understanding the development of Type 2 diabetes mellitus and the process of atherogenesis. Inflammatory markers particularly Tumor necrosis factor-α and high sensitive C-reactive protein interfere with lipid homeostasis and activates proinflammatory mechanisms in type-2 Diabetes mellitus. Our aim of the study was to analyze and correlate the inflammatory markers with lipids in Type2 Diabetes mellitus. 50 diabetic subjects and 50 normal healthy controls were recruited from the outpatient department of Narayana Medical college and hospital, Nellore, A.P). Serum TNF-alpha concentration was measured using Sandwich ELISA kit method. commercial enzymatic methods were used in the determination of serum total.cholesterol, HDL cholesterol and Triacylglycerides. LDL cholesterol was estimated using friedewald equation. Turbidimetric immunoassay (QUANTIA CRP- US) is used for ultrasensitive determination of high sensitive C-reactive protein in serum. Results: serum levels of TNF-α and hs-CRP were significantly elevated in type 2 diabetes mellitus and correlated positively with triacylglycerides, total cholesterol and LDL cholesterol but negatively with HDL cholesterol. We conclude from our study that there is a strong association between proathergenic lipid profile and inflammatory markers in Type2Diabetes mellitus and suggests a significant role of the inflammatory markers in the pathogenesis of dyslipidemia in Type2diabetes mellitus.
ABSTRACT
Obesity and Insulin resistance are very frequent disorders and are described as the dominant risk factors for cardiovascular diseases. Recent research suggests that TNF-alpha, an adipocytokine plays a key role in the development of insulin resistance. Aim of the study was to estimate TNF-alpha levels and to investigate the association of TNF-alpha with the various factors associated with insulin resistance like Body mass index, Waist Hip Ratio, fasting insulin etc. A total of 100 subjects attending out patient department of General Medicine , Narayana Medical College , Nellore were selected for the study.50 patients were diagnosed as type-2 diabetics and 50 subjects were taken as normal healthy controls who attended for a general check-up. Anthropometric and biochemical measurements were analyzed. Serum Insulin and TNF-α were measured using chemiluminescence method and ELISA method respectively. Homeostasis model assessment score was used to gauge the level of insulin resistance. Our Results showed significant elevation in TNF-alpha levels in diabetic subjects and correlations between TNFalpha Body Mass Index and HOMAIR were significant. All correlations were stronger for females compared to males.
ABSTRACT
Oxidative stress has been increasingly implicated in the pathogenesis of cirrhosis. Ethanol and various viral infections will increase the production of reactive oxygen species (ROS) in the liver, resulting in an imbalance between oxidants and antioxidants. Thus determination of oxidants along with antioxidants,stated the role of oxidative stress more accurately in the pathogenesis of liver cirrhosis. In the present study we measured the markers of prooxidants, erythrocyte malondialdehyde (MAD), antioxidants that included erythrocyte catalase, reduced glutathione (GSH), glutathione peroxidase (GPx). 30 subjects with age 25-60 years, who were diagnosed as having liver cirrhosis by the department of Gastroenterology, Narayana Medical Hospital were included, 30 normal healthy individuals of the same age were selected as control . The results clearly indicated that the levels of pro oxidants, MDA were high in cirrhotic subjects than in the controls with p value of 0.0001. The levels of antioxidant enzymes GSH, Catalase were low in cirrhosis with p value of 0.0001 (GSH) and 0.067(Catalase). But the mean value of glutathione peroxidase was high in cirrhosis than in controls. This may be due to conterregulation with oxidative stress. Hence this study indicates the role of oxidative stress in liver cirrhosis and it clearly defines the imbalance between oxidants & antioxidants.